human NPHP1 shRNA silencing Adenovirus

Name 
Ad-h-NPHP1-shRNA
Cat No 
shADV-216958
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human NPHP1.

shADV-216958
Ad-h-NPHP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NPHP1 (nephrocystin 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
JBTS4; NPH1; SLSN1
Description (Vector)
NPHP1 encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell and cell-matrix adhesion signaling, likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1, a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1, also referred to as juvenile nephronophthisis with Leber amaurosis, which is characterized by kidney and eye disease, and with Joubert syndrome type 4, which is characterized by cerebellar ataxia, oculomotor apraxia, psychomotor delay and neonatal breathing abnormalities, sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
4867
Gene Name (Vector)
nephrocystin 1
Gene Symbol
NPHP1
HGNC ID
HGNC:7905
ORF Size (aa)
1845
ORF Size (bp)
1845 bp
RefSeq ID
NM_000272
RefSeq Synonyms
NM_207181, NM_001128179, NM_001128178, NM_000272, BC062574, BC009789,
Species
human
UniGene ID
Hs.280388

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.