human NISCH shRNA silencing Adenovirus

Name 
Ad-h-NISCH-shRNA
Cat No 
shADV-216732
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NISCH.

shADV-216732
Ad-h-NISCH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NISCH (nischarin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
hIRAS; I-1; I-1 receptor candidate protein; I1R; I1R candidate protein; imidazoline receptor 1; imidazoline receptor antisera selected; Imidazoline receptor antisera-selected protein; Imidazoline-1 receptor; Imidazoline-1 receptor candidate protein; IR1; IRAS; nischarin
Description (eCommerce)
Nischarin (NISCH) is a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of NISCH was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation[taken from NCBI Entrez Gene (Gene ID: 11188)].
Gene ID
11188
Gene Name (eCommerce)
nischarin
Gene Name (Vector)
nischarin
Gene Symbol
NISCH
HGNC ID
HGNC:18006
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4515
ORF Size (bp)
4515 bp
Protein Name (eCommerce)
Nischarin
RefSeq ID
NM_007184
RefSeq Synonyms
NM_007184, NM_001276294, NM_001276293, BC056900, BC054494, BC038102,
Research Areas
Apoptosis,Cardiovascular,Cytoskeleton,Metabolism/Metabolic Process,Neurobiology
Research Areas (Faceted)
cardiovascular,cell_biology,metabolism,neurobiology
Species
human
Target Sentence
Nischarin (NISCH) is a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of NISCH was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation[taken from NCBI Entrez Gene (Gene ID: 11188)].
UniGene ID
Hs.435290
UniProt ID (eCommerce)
Q9Y2I1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.