human NIN shRNA silencing Adenovirus

Name 
Ad-h-NIN-shRNA
Cat No 
shADV-216710
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NIN.

shADV-216710
Ad-h-NIN-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NIN (ninein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein; hNinein; ninein; ninein (GSK3B interacting protein); ninein centrosomal protein; SCKL7
Description (eCommerce)
Ninein is a centrosomal protein that associates with the minus-end of microtubules. It serves as an anchor and nucleation point for microtubule elongation from the pericentriolar material (PCM). Ninein also acts as an anchoring protein at apical sites in polarized epithelial cells to assemble non-centrosomal apico-basal arrays.
Description (Vector)
NIN encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Gene ID
51199
Gene Name (eCommerce)
ninein
Gene Name (Vector)
ninein
Gene Symbol
NIN
HGNC ID
HGNC:14906
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4134
ORF Size (bp)
4134 bp
Protein Name (eCommerce)
Ninein
RefSeq ID
NM_016350
RefSeq Synonyms
NM_182946, NM_182944, NM_020921, NM_016350, BC090932,
Research Areas
Cell Cycle,Cell Division,Cytoskeleton,Neurobiology
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,neurobiology
Species
human
Target Sentence
Ninein is a centrosomal protein that associates with the minus-end of microtubules. It serves as an anchor and nucleation point for microtubule elongation from the pericentriolar material (PCM). Ninein also acts as an anchoring protein at apical sites in polarized epithelial cells to assemble non-centrosomal apico-basal arrays.
UniGene ID
Hs.310429
UniProt ID (eCommerce)
Q8N4C6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.