human NHEJ1 shRNA silencing Adenovirus

Name 
Ad-h-NHEJ1-shRNA
Cat No 
shADV-216685
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NHEJ1.

shADV-216685
Ad-h-NHEJ1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NHEJ1 (non-homologous end joining factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
non-homologous end-joining factor 1; nonhomologous end-joining factor 1; protein cernunnos; XLF; XRCC4-like factor
Description (eCommerce)
XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.
Description (Vector)
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
Gene ID
79840
Gene Name (eCommerce)
non-homologous end joining factor 1
Gene Name (Vector)
non-homologous end joining factor 1
Gene Symbol
NHEJ1
HGNC ID
HGNC:25737
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
900
ORF Size (bp)
900 bp
Protein Name (eCommerce)
Non-homologous end-joining factor 1
RefSeq ID
NM_024782
RefSeq Synonyms
NM_024782, BC030986, BC012732,
Research Areas
DNA Damage/Repair,Immunology,Neurobiology,Neurodevelopment
Research Areas (Faceted)
cell_biology,developmental_biology,genetics,immunology,neurobiology,transcription_translation
Species
human
Target Sentence
XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.
UniGene ID
Hs.225988
UniProt ID (eCommerce)
Q9H9Q4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.