human NGLY1 shRNA silencing Adenovirus

Name 
Ad-h-NGLY1-shRNA
Cat No 
shADV-216676
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NGLY1.

shADV-216676
Ad-h-NGLY1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NGLY1 (N-glycanase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CDDG; CDG1V; hPNGase; N-glycanase 1; peptide:N-glycanase; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; PNG1; PNGase
Description (eCommerce)
NGLY1 is an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. NGLY1 may play a role in the proteasome-mediated degradation of misfolded glycoproteins [taken from NCBI Entrez Gene (Gene ID: 55768)].
Description (Vector)
NGLY1 encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
55768
Gene Name (eCommerce)
N-glycanase 1
Gene Name (Vector)
N-glycanase 1
Gene Symbol
NGLY1
HGNC ID
HGNC:17646
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1965
ORF Size (bp)
1965 bp
Protein Name (eCommerce)
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
RefSeq ID
NM_018297
RefSeq Synonyms
NM_018297, NM_001145295, NM_001145294, NM_001145293, BC017220, BC007226,
Research Areas
Cell Biology
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
NGLY1 is an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. NGLY1 may play a role in the proteasome-mediated degradation of misfolded glycoproteins [taken from NCBI Entrez Gene (Gene ID: 55768)].
UniGene ID
Hs.368960
UniProt ID (eCommerce)
Q96IV0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.