human NCOA4 shRNA silencing Adenovirus

Name 
Ad-h-NCOA4-shRNA
Cat No 
shADV-216422
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NCOA4.

shADV-216422
Ad-h-NCOA4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NCOA4 (nuclear receptor coactivator 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
70 kDa androgen receptor coactivator; 70 kDa AR-activator; Androgen receptor coactivator 70 kDa protein; androgen receptor-associated protein of 70 kDa; ARA70; ELE1; NCoA-4; nuclear receptor coactivator 4; PTC3; ret fused; RET-activating gene ELE1; Ret-activating protein ELE1; RFG
Description (eCommerce)
ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.
Description (Vector)
NCOA4 encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.
Gene ID
8031
Gene Name (eCommerce)
nuclear receptor coactivator 4
Gene Name (Vector)
nuclear receptor coactivator 4
Gene Symbol
NCOA4
HGNC ID
HGNC:7671
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1728
ORF Size (bp)
1728 bp
Protein Name (eCommerce)
Nuclear receptor coactivator 4
RefSeq ID
NM_005437
RefSeq Synonyms
NM_005437, NM_001145263, NM_001145262, NM_001145261, NM_001145260, BC012736, BC001562,
Research Areas
Cancer,Nuclear Receptor Coactivators,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cell_biology,transcription_translation
Species
human
Target Sentence
ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.
UniGene ID
Hs.709644
UniProt ID (eCommerce)
Q13772

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.