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human MED8 shRNA silencing Adenovirus

Name 
Ad-h-MED8-shRNA
Cat No 
shADV-215267
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human MED8.

shADV-215267
Ad-h-MED8-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MED8 (mediator complex subunit 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ARC32
Description (Vector)
MED8 encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
Gene ID
112950
Gene Name (Vector)
mediator complex subunit 8
Gene Symbol
MED8
HGNC ID
HGNC:19971
ORF Size (aa)
540
RefSeq ID
NM_052877
RefSeq Synonyms
NM_201542, NM_052877, NM_001001653, BC010019,
Species
human
UniGene ID
Hs.301756

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.