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human MCAM shRNA silencing Adenovirus

Name 
Ad-h-MCAM-shRNA
Cat No 
shADV-215147
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MCAM.

shADV-215147
Ad-h-MCAM-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MCAM (melanoma cell adhesion molecule). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CD antigen CD146; CD146; cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; melanoma adhesion molecule; Melanoma cell adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18; MelCAM; METCAM; MUC18; S-endo 1 endothelial-associated antigen
Description (eCommerce)
CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P43121].
Gene ID
4162
Gene Name (eCommerce)
melanoma cell adhesion molecule
Gene Name (Vector)
melanoma cell adhesion molecule
Gene Symbol
MCAM
HGNC ID
HGNC:6934
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1941
ORF Size (bp)
1941 bp
Protein Name (eCommerce)
Cell surface glycoprotein MUC18
RefSeq ID
NM_006500
RefSeq Synonyms
NM_006500, BC056418,
Research Areas
Immunology,Signal Transduction
Research Areas (Faceted)
signal_transduction,immunology
Species
human
Target Sentence
CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P43121].
UniGene ID
Hs.599039
UniProt ID (eCommerce)
P43121

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.