human MBD2 shRNA silencing Adenovirus

Name 
Ad-h-MBD2-shRNA
Cat No 
shADV-215088
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MBD2.

shADV-215088
Ad-h-MBD2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MBD2 (methyl-CpG binding domain protein 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
demethylase; DMTase; methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41
Description (eCommerce)
MBD2 (methyl-CpG-binding domain protein 2) binds methylated CpG sites and represses gene transcription. DNA methylation is a common mechanism of gene silencing and is central to the developmental process. Methyl-CpG binding proteins (MBPs) have a high affinity for methylated DNA and are proposed to mediate gene silencing via the recruitment of histone deacetylases and other transcriptional repressors to chromatin.
Description (Vector)
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
8932
Gene Name (eCommerce)
methyl-CpG binding domain protein 2
Gene Name (Vector)
methyl-CpG binding domain protein 2
Gene Symbol
MBD2
HGNC ID
HGNC:6917
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1236
ORF Size (bp)
1236 bp
Protein Name (eCommerce)
Methyl-CpG-binding domain protein 2
RefSeq ID
NM_003927
RefSeq Synonyms
NM_015832, NM_003927, BC032638,
Research Areas
Epigenetic Regulation of Gene Expression,Gene Expression,mRNA Binding,RNA Binding,Signal Transduction,Transcription Factor/Regulator,Wnt Signaling
Research Areas (Faceted)
cell_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
MBD2 (methyl-CpG-binding domain protein 2) binds methylated CpG sites and represses gene transcription. DNA methylation is a common mechanism of gene silencing and is central to the developmental process. Methyl-CpG binding proteins (MBPs) have a high affinity for methylated DNA and are proposed to mediate gene silencing via the recruitment of histone deacetylases and other transcriptional repressors to chromatin.
UniGene ID
Hs.25674
UniProt ID (eCommerce)
Q9UBB5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.