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human LIG3 shRNA silencing Adenovirus

Name 
Ad-h-LIG3-shRNA
Cat No 
shADV-213922
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human LIG3.

shADV-213922
Ad-h-LIG3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human LIG3 (DNA ligase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA ligase 3; DNA ligase III; LIG2; LIG3alpha; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase ATP 3
Description (eCommerce)
DNA ligase III catalyzes the joining of DNA ends during DNA strand-break repair. DNA ligase III interacts with the DNA-repair factor XRCC1 to function in base excision repair (BER). DNA ligase III has also been shown to play a required role in microhomology-mediated end-joining of double-strand breaks.
Description (Vector)
LIG3 is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID
3980
Gene Name (eCommerce)
DNA ligase 3
Gene Name (Vector)
DNA ligase 3
Gene Symbol
LIG3
HGNC ID
HGNC:6600
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3030
ORF Size (bp)
3030 bp
Protein Name (eCommerce)
DNA ligase 3
RefSeq ID
NM_002311
RefSeq Synonyms
NM_013975, NM_002311, BC068005, BC009026,
Research Areas
Cell Cycle,Cell Division,DNA Damage/Repair,DNA Replication,Mitochondrion,Spermatogenesis,Zinc-finger
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation
Species
human
Target Sentence
DNA ligase III catalyzes the joining of DNA ends during DNA strand-break repair. DNA ligase III interacts with the DNA-repair factor XRCC1 to function in base excision repair (BER). DNA ligase III has also been shown to play a required role in microhomology-mediated end-joining of double-strand breaks.
UniGene ID
Hs.100299
UniProt ID (eCommerce)
P49916

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.