human HYOU1 shRNA silencing Adenovirus

Name 
Ad-h-HYOU1-shRNA
Cat No 
shADV-211734
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human HYOU1.

shADV-211734
Ad-h-HYOU1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human HYOU1 (hypoxia up-regulated 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; epididymis secretory sperm binding protein; Grp170; GRP-170; HSP12A; hypoxia up-regulated protein 1; ORP150; ORP-150; oxygen regulated protein (150kD)
Description (eCommerce)
ORP150 belongs to the heat shock protein 70 family that is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. ORP150 is also thought to play an important role in protein folding and secretion in the ER and is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. Finally, ORP150 has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. [taken from NCBI Entrez Gene (Gene ID: 10525)].
Description (Vector)
HYOU1 belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. HYOU1 is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
Gene ID
10525
Gene Name (eCommerce)
hypoxia up-regulated 1
Gene Name (Vector)
hypoxia up-regulated 1
Gene Symbol
HYOU1
HGNC ID
HGNC:16931
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2037
ORF Size (bp)
2037 bp
Protein Name (eCommerce)
Hypoxia up-regulated protein 1
RefSeq ID
NM_006389
RefSeq Synonyms
NM_006389, NM_001130991, BC072436, BC004560,
Research Areas
Hypoxia,Signal Transduction
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
ORP150 belongs to the heat shock protein 70 family that is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. ORP150 is also thought to play an important role in protein folding and secretion in the ER and is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. Finally, ORP150 has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. [taken from NCBI Entrez Gene (Gene ID: 10525)].
UniGene ID
Hs.277704
UniProt ID (eCommerce)
Q9Y4L1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.