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human HSD3B2 shRNA silencing Adenovirus

Name 
Ad-h-HSD3B2-shRNA
Cat No 
shADV-211584
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human HSD3B2.

shADV-211584
Ad-h-HSD3B2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human HSD3B2 (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HSD3B; HSDB; SDR11E2
Description (Vector)
HSD3B2 is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
Gene ID
3284
Gene Name (Vector)
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Gene Symbol
HSD3B2
HGNC ID
HGNC:5218
ORF Size (aa)
1119
ORF Size (bp)
1119 bp
RefSeq ID
NM_000198
RefSeq Synonyms
NM_001166120, NM_000198, BC131488, BC038419,
Species
human
UniGene ID
Hs.654399

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.