human HMGCR shRNA silencing Adenovirus

Name 
Ad-h-HMGCR-shRNA
Cat No 
shADV-211283
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human HMGCR.

shADV-211283
Ad-h-HMGCR-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human HMGCR (3-hydroxy-3-methylglutaryl-CoA reductase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
LDLCQ3
Description (Vector)
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
3156
Gene Name (Vector)
3-hydroxy-3-methylglutaryl-CoA reductase
Gene Symbol
HMGCR
HGNC ID
HGNC:5006
ORF Size (aa)
2508
ORF Size (bp)
2508 bp
RefSeq ID
NM_000859
RefSeq Synonyms
NM_001130996, NM_000859, BC033692,
Species
human
UniGene ID
Hs.713530

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.