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human H2AFX shRNA silencing Adenovirus

Name 
Ad-h-H2AFX-shRNA
Cat No 
shADV-210742
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human H2AFX.

shADV-210742
Ad-h-H2AFX-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human H2AFX (H2A histone family member X). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; histone H2AX
Description (eCommerce)
H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.
Description (Vector)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Gene ID
3014
Gene Name (eCommerce)
H2A histone family member X
Gene Name (Vector)
H2A histone family member X
Gene Symbol
H2AFX
HGNC ID
HGNC:4739
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
432
Protein Name (eCommerce)
Histone H2AX
RefSeq ID
NM_002105
RefSeq Synonyms
NM_002105, BC013416, BC011694, BC004915,
Research Areas
Cell Cycle,Complexome,DNA Damage/Repair,Fanconi anemia,Gene Expression,Homologous Recombination,Meiosis,Phospho-specific,Proteasome,Replication,Spermatogenesis,Telomeric Maintenance
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation
Species
human
Target Sentence
H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.
UniGene ID
Hs.477879
UniProt ID (eCommerce)
P16104

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.