human EEF1A1 shRNA silencing Adenovirus

Name
Ad-h-EEF1A1-shRNA

Cat No
shADV-207574

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human EEF1A1.

shADV-207574

Ad-h-EEF1A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human EEF1A1 (eukaryotic translation elongation factor 1 alpha 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1

Description (Vector)

EEF1A1 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. EEF1A1 has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

Gene ID

1915

Gene Name (Vector)

eukaryotic translation elongation factor 1 alpha 1

Gene Symbol

EEF1A1

HGNC ID

HGNC:3189

ORF Size (aa)

1389

ORF Size (bp)

1389 bp

RefSeq ID

NM_001402

RefSeq Synonyms

NM_001402, BC111051, BC094687, BC082268, BC072385, BC071841, BC071741, BC071727, BC071619, BC066893, BC057391, BC038339, BC028674, BC021686, BC018641, BC018150, BC014224, BC012891, BC010735, BC009875, BC009733, BC008587,

Species

human

UniGene ID

Hs.713109

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.