human DTL shRNA silencing Adenovirus

Name 
Ad-h-DTL-shRNA
Cat No 
shADV-207370
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human DTL.

shADV-207370
Ad-h-DTL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DTL (denticleless E3 ubiquitin protein ligase homolog). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CDT2; DCAF2; DDB1- and CUL4-associated factor 2; denticleless protein homolog; L2DTL; lethal(2) denticleless protein homolog; RAMP; RA-regulated nuclear matrix-associated protein; retinoic acid-regulated nuclear matrix-associated protein
Description (eCommerce)
DTL/CDT2 is a component of the CUL4A-DDB1 E3 ubiquitinating complex and plays a role in downregulating CDT1, an essential component of pre-replication complexes (Pre-RC) that ensures the genome is replicated only once per cycle. As part of the CUL4A-DDB1 E3 ligase complex DTL/CDT2 is also involved in the regulation of other proteins involved in the G2/M cell cycle checkpoint.
Gene ID
51514
Gene Name (eCommerce)
denticleless E3 ubiquitin protein ligase homolog
Gene Name (Vector)
denticleless E3 ubiquitin protein ligase homolog
Gene Symbol
DTL
HGNC ID
HGNC:30288
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2193
ORF Size (bp)
2193 bp
Protein Name (eCommerce)
Denticleless protein homolog
RefSeq ID
NM_016448
RefSeq Synonyms
NM_016448, NM_001286230, NM_001286229, BC033540, BC033297,
Research Areas
Cell Cycle,Cytoskeleton,DNA Damage/Repair,DNA Replication,Ubiquitination
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
DTL/CDT2 is a component of the CUL4A-DDB1 E3 ubiquitinating complex and plays a role in downregulating CDT1, an essential component of pre-replication complexes (Pre-RC) that ensures the genome is replicated only once per cycle. As part of the CUL4A-DDB1 E3 ligase complex DTL/CDT2 is also involved in the regulation of other proteins involved in the G2/M cell cycle checkpoint.
UniGene ID
Hs.656473
UniProt ID (eCommerce)
Q9NZJ0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.