human DPH5 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human DPH5.
shADV-207278
Ad-h-DPH5-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human DPH5 (diphthamide biosynthesis 5). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AD-018; CGI-30; HSPC143; NPD015
Description (Vector)
DPH5 encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
51611
Gene Name (Vector)
diphthamide biosynthesis 5
Gene Symbol
DPH5
HGNC ID
HGNC:24270
ORF Size (aa)
858
ORF Size (bp)
858 bp
RefSeq ID
NM_015958
RefSeq Synonyms
NM_015958, NM_001077395, NM_001077394, BC053857, BC009620,
Species
human
UniGene ID
Hs.440776
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.