human DPAGT1 shRNA silencing Adenovirus

Name 
Ad-h-DPAGT1-shRNA
Cat No 
shADV-207263
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human DPAGT1.

shADV-207263
Ad-h-DPAGT1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DPAGT1 (dolichyl-phosphate N-acetylglucosaminephosphotransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT
Description (Vector)
DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme.
Gene ID
1798
Gene Name (Vector)
dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Gene Symbol
DPAGT1
HGNC ID
HGNC:2995
ORF Size (aa)
906
ORF Size (bp)
906 bp
RefSeq ID
NM_001382
RefSeq Synonyms
NM_001382, BC047771, BC000325,
Species
human
UniGene ID
Hs.524081

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.