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human DCTN2 shRNA silencing Adenovirus

Name 
Ad-h-DCTN2-shRNA
Cat No 
shADV-206673
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human DCTN2.

shADV-206673
Ad-h-DCTN2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DCTN2 (dynactin subunit 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
50 kDa dynein-associated polypeptide; DCTN50; DCTN-50; dynactin 2 (p50); dynactin complex 50 kDa subunit; dynactin subunit 2; DYNAMITIN; epididymis secretory protein Li 77; epididymis secretory sperm binding protein; HEL-S-77; p50 dynamitin; RBP50
Description (eCommerce)
Dynactin subunit 2 (DCTN2) modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. DCTN2 is involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13561].
Description (Vector)
DCTN2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene ID
10540
Gene Name (eCommerce)
dynactin subunit 2
Gene Name (Vector)
dynactin subunit 2
Gene Symbol
DCTN2
HGNC ID
HGNC:2712
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1206
ORF Size (bp)
1206 bp
Protein Name (eCommerce)
Dynactin subunit 2
RefSeq ID
NM_006400
RefSeq Synonyms
NM_006400, NM_001261413, NM_001261412, BC014083, BC009468, BC000718,
Research Areas
Antigen Processing and Presentation,Cell Cycle,Cell Division,Cytoskeleton,Huntington's Disease,Immunology,Neurobiology,Neurodegeneration,Neurodevelopment
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,immunology,neurobiology
Species
human
Target Sentence
Dynactin subunit 2 (DCTN2) modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. DCTN2 is involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13561].
UniGene ID
Hs.289123
UniProt ID (eCommerce)
Q13561

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.