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human CUL3 shRNA silencing Adenovirus

Name 
Ad-h-CUL3-shRNA
Cat No 
shADV-206299
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CUL3.

shADV-206299
Ad-h-CUL3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CUL3 (cullin 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CUL-3; cullin-3; PHA2E
Description (eCommerce)
Cul3 is a member of the cullin group of proteins and functions as a scaffold to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. Cul3 and its associated ubiquitin ligase activity are essential for mitotic division and required for correct alignment of chromosomes and the completion of cytokinesis. Aurora B is a substrate of Cul3-based E3 ubiquitin ligase activity.
Description (Vector)
CUL3 encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
8452
Gene Name (eCommerce)
cullin 3
Gene Name (Vector)
cullin 3
Gene Symbol
CUL3
HGNC ID
HGNC:2553
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2307
ORF Size (bp)
2307 bp
Protein Name (eCommerce)
Cullin-3
RefSeq ID
NM_003590
RefSeq Synonyms
NM_003590, NM_001257198, NM_001257197, BC092409, BC039598,
Research Areas
Antigen Processing and Presentation,Apoptosis,Cell Cycle,Golgi Apparatus,Immunology,Rho Signaling,Signal Transduction,Ubiquitination,Wnt Signaling
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,immunology,signal_transduction
Species
human
Target Sentence
Cul3 is a member of the cullin group of proteins and functions as a scaffold to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. Cul3 and its associated ubiquitin ligase activity are essential for mitotic division and required for correct alignment of chromosomes and the completion of cytokinesis. Aurora B is a substrate of Cul3-based E3 ubiquitin ligase activity.
UniGene ID
Hs.372286
UniProt ID (eCommerce)
Q13618

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.