human CROP shRNA silencing Adenovirus

Name 
Ad-h-CROP-shRNA
Cat No 
shADV-206032
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human LUC7L3.

shADV-206032
Ad-h-CROP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human LUC7L3 (LUC7 like 3 pre-mRNA splicing factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
cAMP regulatory element-associated protein 1; cisplatin resistance-associated-overexpressed protein; CRA; CREAP-1; CRE-associated protein 1; CROP; hLuc7A; LUC7A; LUC7-like 3; luc7-like protein 3; OA48-18; okadaic acid-inducible phosphoprotein OA48-18
Description (eCommerce)
Cisplatin resistance-associated overexpressed protein (CROP) was originally cloned by differential display from cisplatin-resistant cells. It is an SR-related protein and the human homolog of the yeast protein, Luc7p. Luc7p is an essential protein involved in vegetative growth and 5’-splice recognition. CROP/Luc7a contains arginine- and glutamate-rich domains (RE domains) and arginine- and serine-rich domains (RS domains). It localizes to the nucleus and is distributed as speckles. CROP/Luc7a has been shown to interact with SF2/ASF, a protein involved in RNA splicing. Due to this association and the known function of its yeast homolog, CROP/Luc7a is proposed to play a role in RNA splicing.
Description (Vector)
LUC7L3 encodes a protein with an N-terminal half that contains cysteine/histidine motifs and leucine zipper-like repeats, and the C-terminal half is rich in arginine and glutamate residues (RE domain) and arginine and serine residues (RS domain). This protein localizes with a speckled pattern in the nucleus, and could be involved in the formation of splicesome via the RE and RS domains. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID
51747
Gene Name (eCommerce)
LUC7 like 3 pre-mRNA splicing factor
Gene Name (Vector)
LUC7 like 3 pre-mRNA splicing factor
Gene Symbol
LUC7L3
HGNC ID
HGNC:24309
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1299
ORF Size (bp)
1299 bp
Protein Name (eCommerce)
Luc7-like protein 3
RefSeq ID
NM_006107
RefSeq Synonyms
NM_016424, NM_006107, NM_001330330, BC156954, BC056409,
Research Areas
Apoptosis,mRNA Binding,mRNA Processing,mRNA Splicing,RNA Processing
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
Cisplatin resistance-associated overexpressed protein (CROP) was originally cloned by differential display from cisplatin-resistant cells. It is an SR-related protein and the human homolog of the yeast protein, Luc7p. Luc7p is an essential protein involved in vegetative growth and 5’-splice recognition. CROP/Luc7a contains arginine- and glutamate-rich domains (RE domains) and arginine- and serine-rich domains (RS domains). It localizes to the nucleus and is distributed as speckles. CROP/Luc7a has been shown to interact with SF2/ASF, a protein involved in RNA splicing. Due to this association and the known function of its yeast homolog, CROP/Luc7a is proposed to play a role in RNA splicing.
UniGene ID
Hs.130293
UniProt ID (eCommerce)
O95232

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.