human CENPB shRNA silencing Adenovirus

Name 
Ad-h-CENPB-shRNA
Cat No 
shADV-204951
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CENPB.

shADV-204951
Ad-h-CENPB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CENPB (centromere protein B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CENP-B; centromere autoantigen B; Centromere protein B; centromere protein B, 80kDa; major centromere autoantigen B
Description (eCommerce)
CENP-B is a centromeric protein that specifically binds the CENP-B box, a 17-base pair DNA sequence found in a subset of alpha-satellite DNA repeats. CENP-B associates with CENP-A and CENP-C in centromere specific nucleosomes to form the pre-kinetochore. CENP-B auto-Antibodies have been identified in sera from patients with autoimmune diseases.
Description (Vector)
CENPB product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Gene ID
1059
Gene Name (eCommerce)
centromere protein B
Gene Name (Vector)
centromere protein B
Gene Symbol
CENPB
HGNC ID
HGNC:1852
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1800
ORF Size (bp)
1800 bp
Protein Name (eCommerce)
Major centromere autoantigen B
RefSeq ID
NM_001810
RefSeq Synonyms
NM_001810, BC053847,
Research Areas
Chromatin Remodeling,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
CENP-B is a centromeric protein that specifically binds the CENP-B box, a 17-base pair DNA sequence found in a subset of alpha-satellite DNA repeats. CENP-B associates with CENP-A and CENP-C in centromere specific nucleosomes to form the pre-kinetochore. CENP-B auto-Antibodies have been identified in sera from patients with autoimmune diseases.
UniGene ID
Hs.713340
UniProt ID (eCommerce)
P07199

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.