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human CCDC88A shRNA silencing Adenovirus

Name 
Ad-h-CCDC88A-shRNA
Cat No 
shADV-204384
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CCDC88A.

shADV-204384
Ad-h-CCDC88A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CCDC88A (coiled-coil domain containing 88A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Akt phosphorylation enhancer; AKT-phosphorylation enhancer; APE; Coiled-coil domain-containing protein 88A; g alpha-interacting vesicle-associated protein; girders of actin filament; girders of actin filaments; GIRDIN; GIV; GRDN; HkRP1; Hook-related protein 1; KIAA1212; PEHO; PEHOL
Description (eCommerce)
CCDC88A encodes the protein known as GIV (G alpha-interacting vesicle-associated protein) and Girdin. CCDC88A plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. CCDC88A Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. CCDC88A has also been found to be essential for the integrity of the actin cytoskeleton and for cell migration. It is required for formation of actin stress fibers and lamellipodia, and it may be involved in membrane sorting in the early endosome [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q3V6T2].
Description (Vector)
CCDC88A encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
55704
Gene Name (eCommerce)
coiled-coil domain containing 88A
Gene Name (Vector)
coiled-coil domain containing 88A
Gene Symbol
CCDC88A
HGNC ID
HGNC:25523
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5532
ORF Size (bp)
5532 bp
Protein Name (eCommerce)
Girdin
RefSeq ID
NM_018084
RefSeq Synonyms
NM_018084, NM_001254943, NM_001135597, BC144320, BC132736,
Research Areas
Cytoskeleton,DNA Replication,Golgi Apparatus,mTOR Signaling,Neurobiology,Neurodevelopment,Neurogenesis,Signal Transduction
Research Areas (Faceted)
cell_biology,developmental_biology,genetics,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
CCDC88A encodes the protein known as GIV (G alpha-interacting vesicle-associated protein) and Girdin. CCDC88A plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. CCDC88A Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. CCDC88A has also been found to be essential for the integrity of the actin cytoskeleton and for cell migration. It is required for formation of actin stress fibers and lamellipodia, and it may be involved in membrane sorting in the early endosome [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q3V6T2].
UniGene ID
Hs.292925
UniProt ID (eCommerce)
Q3V6T2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.