human CAST shRNA silencing Adenovirus

Name 
Ad-h-CAST-shRNA
Cat No 
shADV-204134
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CAST.

shADV-204134
Ad-h-CAST-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CAST (calpastatin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BS-17; calpain inhibitor; calpastatin; PLACK; sperm BS-17 component
Description (eCommerce)
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation [taken from NCBI Entrez Gene (Gene ID: 831)].
Description (Vector)
CAST is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID
831
Gene Name (eCommerce)
calpastatin
Gene Name (Vector)
calpastatin
Gene Symbol
CAST
HGNC ID
HGNC:1515
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2004
ORF Size (bp)
2004 bp
Protein Name (eCommerce)
Calpastatin
RefSeq ID
NM_173060
RefSeq Synonyms
NM_173060, NM_001750, NM_001330634, NM_001330633, NM_001330632, NM_001330631, NM_001330630, NM_001330629, NM_001330628, NM_001330627, NM_001330626, NM_001284213, NM_001284212, NM_001190442, NM_001042446, NM_001042445, NM_001042444, NM_001042443, NM_001042442, NM_001042441, NM_001042440, BC013579,
Research Areas
Cell Biology,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation [taken from NCBI Entrez Gene (Gene ID: 831)].
UniGene ID
Hs.436186
UniProt ID (eCommerce)
P20810

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.