human ATP2A1 shRNA silencing Adenovirus

Name 
Ad-h-ATP2A1-shRNA
Cat No 
shADV-201691
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human ATP2A1.

shADV-201691
Ad-h-ATP2A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATP2A1 (ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATP2A; SERCA1
Description (Vector)
ATP2A1 encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.
Gene ID
487
Gene Name (Vector)
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
Gene Symbol
ATP2A1
HGNC ID
HGNC:811
ORF Size (aa)
3003
ORF Size (bp)
3003 bp
RefSeq ID
NM_004320
RefSeq Synonyms
NM_173201, NM_004320, NM_001286075, BC148653,
Species
human
UniGene ID
Hs.657344

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.