human ATG12 shRNA silencing Adenovirus

Name 
Ad-h-ATG12-shRNA
Cat No 
shADV-201633
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ATG12.

shADV-201633
Ad-h-ATG12-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATG12 (autophagy related 12). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
APG12; APG12L; FBR93; HAPG12
Description (Vector)
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 (PubMed 9852036)).(supplied by OMIM, Mar 2008)
Gene ID
9140
Gene Name (Vector)
autophagy related 12
Gene Symbol
ATG12
HGNC ID
HGNC:588
ORF Size (aa)
564
ORF Size (bp)
564 bp
RefSeq ID
NM_004707
RefSeq Synonyms
NM_004707, NM_001277783, BC012266,
Species
human
UniGene ID
Hs.264482

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.