human ASPH shRNA silencing Adenovirus

Name 
Ad-h-ASPH-shRNA
Cat No 
shADV-201573
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ASPH.

shADV-201573
Ad-h-ASPH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ASPH (aspartate beta-hydroxylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A beta H-J-J; AAH; ASP beta-hydroxylase; Aspartate beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; cardiac junctin; CASQ2BP1; FDLAB; HAAH; humbug; JCTN; junctate; junctin; peptide-aspartate beta-dioxygenase
Description (eCommerce)
Aspartyl/asparaginyl beta-hydroxyl (ASPH) Isoform 1 specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12797].
Description (Vector)
ASPH is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.
Gene ID
444
Gene Name (eCommerce)
aspartate beta-hydroxylase
Gene Name (Vector)
aspartate beta-hydroxylase
Gene Symbol
ASPH
HGNC ID
HGNC:757
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2277
ORF Size (bp)
2277 bp
Protein Name (eCommerce)
Aspartyl/asparaginyl beta-hydroxylase
RefSeq ID
NM_004318
RefSeq Synonyms
NM_032468, NM_032467, NM_032466, NM_020164, NM_004318, NM_001164756, NM_001164755, NM_001164754, NM_001164753, NM_001164752, NM_001164751, NM_001164750, BC166658, BC025236,
Research Areas
Hypoxia
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Aspartyl/asparaginyl beta-hydroxyl (ASPH) Isoform 1 specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12797].
UniGene ID
Hs.591874
UniProt ID (eCommerce)
Q12797

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.