human ARFGAP1 shRNA silencing Adenovirus

Name 
Ad-h-ARFGAP1-shRNA
Cat No 
shADV-201282
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ARFGAP1.

shADV-201282
Ad-h-ARFGAP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ARFGAP1 (ADP ribosylation factor GTPase activating protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; HRIHFB2281
Description (eCommerce)
ADP-ribosylation factor GTPase activating protein 1 (ArfGAP1) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. ArfGAP1 promotes the hydrolysis of ARF1-bound GTP to promote fusion of golgi-derived vesicles with the target compartment. Although they are differentially regulated, ArfGAP1, ArfGAP2, and ArfGAP3 have been shown to play overlapping roles in COPI-mediated transport and in the maintenance of Golgi organization.
Description (Vector)
ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
55738
Gene Name (eCommerce)
ADP ribosylation factor GTPase activating protein 1
Gene Name (Vector)
ADP ribosylation factor GTPase activating protein 1
Gene Symbol
ARFGAP1
HGNC ID
HGNC:15852
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1245
ORF Size (bp)
1245 bp
Protein Name (eCommerce)
ADP-ribosylation factor GTPase-activating protein 1
RefSeq ID
NM_175609
RefSeq Synonyms
NM_175609, NM_018209, NM_001281484, NM_001281483, NM_001281482, BC028233, BC011876, BC006085, BC000786,
Research Areas
Golgi Apparatus,Immunology,RAC1 Signaling,Rho Signaling,Signal Transduction,T-cell Receptor Signaling,Transport,Zinc-finger
Research Areas (Faceted)
signal_transduction,cell_biology,immunology,signal_transduction,cell_biology
Species
human
Target Sentence
ADP-ribosylation factor GTPase activating protein 1 (ArfGAP1) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. ArfGAP1 promotes the hydrolysis of ARF1-bound GTP to promote fusion of golgi-derived vesicles with the target compartment. Although they are differentially regulated, ArfGAP1, ArfGAP2, and ArfGAP3 have been shown to play overlapping roles in COPI-mediated transport and in the maintenance of Golgi organization.
UniGene ID
Hs.25584
UniProt ID (eCommerce)
Q8N6T3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.