human ALDH18A1 shRNA silencing Adenovirus

Name 
Ad-h-ALDH18A1-shRNA
Cat No 
shADV-200724
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ALDH18A1.

shADV-200724
Ad-h-ALDH18A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ALDH18A1 (aldehyde dehydrogenase 18 family member A1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ADCL3; aldehyde dehydrogenase family 18 member A1; ARCL3A; delta1-pyrroline-5-carboxlate synthetase; delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase (GPR); Glutamate 5-kinase (GK); Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSAS; P5CS; PYCS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); spastic paraplegia 9 (autosomal dominant); Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux); SPG9; SPG9A; SPG9B
Description (eCommerce)
ALDH18A1/P5CS is a bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P54886].
Description (Vector)
ALDH18A1 is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.
Gene ID
5832
Gene Name (eCommerce)
aldehyde dehydrogenase 18 family member A1
Gene Name (Vector)
aldehyde dehydrogenase 18 family member A1
Gene Symbol
ALDH18A1
HGNC ID
HGNC:9722
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2382
ORF Size (bp)
2382 bp
Protein Name (eCommerce)
Delta-1-pyrroline-5-carboxylate synthase
RefSeq ID
NM_002860
RefSeq Synonyms
NM_002860, NM_001323419, NM_001323418, NM_001323417, NM_001323416, NM_001323415, NM_001323414, NM_001323413, NM_001323412, NM_001017423, BC143930, BC117242, BC117240, BC106054,
Research Areas
Metabolism/Metabolic Process,Mitochondrion,Neurobiology
Research Areas (Faceted)
cell_biology,metabolism,neurobiology
Species
human
Target Sentence
ALDH18A1/P5CS is a bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P54886].
UniGene ID
Hs.500645
UniProt ID (eCommerce)
P54886

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.