human ALAS1 shRNA silencing Adenovirus

Name 
Ad-h-ALAS1-shRNA
Cat No 
shADV-200713
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ALAS1.

shADV-200713
Ad-h-ALAS1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ALAS1 (5′-aminolevulinate synthase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALAS; ALAS-H; ALAS3; ALASH; MIG4
Description (Vector)
ALAS1 encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Gene ID
211
Gene Name (Vector)
5'-aminolevulinate synthase 1
Gene Symbol
ALAS1
HGNC ID
HGNC:396
ORF Size (aa)
1923
ORF Size (bp)
1923 bp
RefSeq ID
NM_000688
RefSeq Synonyms
NM_199166, NM_001304444, NM_001304443, NM_000688, BC011798,
Species
human
UniGene ID
Hs.476308

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.