human ALAD shRNA silencing Adenovirus

Name 
Ad-h-ALAD-shRNA
Cat No 
shADV-200712
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ALAD.

shADV-200712
Ad-h-ALAD-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ALAD (aminolevulinate dehydratase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALADH; PBGS
Description (Vector)
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.
Gene ID
210
Gene Name (Vector)
aminolevulinate dehydratase
Gene Symbol
ALAD
HGNC ID
HGNC:395
ORF Size (aa)
1020
RefSeq ID
NM_000031
RefSeq Synonyms
NM_001317745, NM_001003945, NM_000031, BC000977,
Species
human
UniGene ID
Hs.1227

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.