human ADA shRNA silencing Adenovirus

Name 
Ad-h-ADA-shRNA
Cat No 
shADV-200340
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ADA.

shADV-200340
Ad-h-ADA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ADA (adenosine deaminase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
adenosine aminohydrolase; adenosine deaminase
Description (eCommerce)
The gene ADA encodes the enzyme adenosine deaminase which catalyzes the hydrolysis of adenosine to inosine. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins. Elevated levels of this enzyme have been associated with congenital hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 100)].
Description (Vector)
ADA encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.
Gene ID
100
Gene Name (eCommerce)
adenosine deaminase
Gene Name (Vector)
adenosine deaminase
Gene Symbol
ADA
HGNC ID
HGNC:186
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1092
ORF Size (bp)
1092 bp
Protein Name (eCommerce)
Adenosine deaminase
RefSeq ID
NM_000022
RefSeq Synonyms
NM_001322051, NM_001322050, NM_000022, BC040226, BC007678,
Research Areas
Aging,Apoptosis,Cancer,Cardiology,Cardiovascular,Hypoxia,Immunology,Lysosome,Metabolism/Metabolic Process,Neurobiology,T-cell Receptor Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,immunology,metabolism,neurobiology,signal_transduction
Species
human
Target Sentence
The gene ADA encodes the enzyme adenosine deaminase which catalyzes the hydrolysis of adenosine to inosine. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins. Elevated levels of this enzyme have been associated with congenital hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 100)].
UniGene ID
Hs.654536
UniProt ID (eCommerce)
P00813

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.