human AATF shRNA silencing Adenovirus

Name 
Ad-h-AATF-shRNA
Cat No 
shADV-200038
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human AATF.

shADV-200038
Ad-h-AATF-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human AATF (apoptosis antagonizing transcription factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Apoptosis-antagonizing transcription factor; BFR2; CHE1; CHE-1; DED; protein AATF; rb-binding protein Che-1
Description (eCommerce)
AATF/Che-1 was identified as a protein that interacts with RNA polymerase II and is involved in the regulation of gene transcription and cell proliferation. AATF/Che-1 has been demonstrated to promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) and interfering with HDAC1’s interaction with the growth inhibiting protein pRB. AATF has also been shown to function as a nuclear receptor coactivator and a repressor of apoptosis.
Description (Vector)
AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
Gene ID
26574
Gene Name (eCommerce)
apoptosis antagonizing transcription factor
Gene Name (Vector)
apoptosis antagonizing transcription factor
Gene Symbol
AATF
HGNC ID
HGNC:19235
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1683
ORF Size (bp)
1683 bp
Protein Name (eCommerce)
Protein AATF
RefSeq ID
NM_012138
RefSeq Synonyms
NM_012138, BC000591,
Research Areas
Apoptosis,Cell Cycle,DNA Damage/Repair,Golgi Apparatus,Neurobiology,NGF Signaling,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,neurobiology,signal_transduction
Species
human
Target Sentence
AATF/Che-1 was identified as a protein that interacts with RNA polymerase II and is involved in the regulation of gene transcription and cell proliferation. AATF/Che-1 has been demonstrated to promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) and interfering with HDAC1’s interaction with the growth inhibiting protein pRB. AATF has also been shown to function as a nuclear receptor coactivator and a repressor of apoptosis.
UniGene ID
Hs.195740
UniProt ID (eCommerce)
Q9NY61

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.