rat GALNAC4S-6ST shRNA silencing AAV

Name
AAV-r-GALNAC4S-6ST-shRNA

Cat No
shAAV-293761

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Chst15.

shAAV-293761

AAV-r-GALNAC4S-6ST-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Chst15 (carbohydrate sulfotransferase 15). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Galnac4s-6st; GalNAc4S6ST

Description (Vector)

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Gene ID

286974

Gene Name (Vector)

carbohydrate sulfotransferase 15

Gene Symbol

Chst15

ORF Size (aa)

1212

ORF Size (bp)

1212 bp

RefSeq ID

NM_173310

RefSeq Synonyms

NM_173310, BC085687,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.