rat H2AFY shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat H2afy.
shAAV-293158
AAV-r-H2AFY-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat H2afy (H2A histone family, member Y). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
Gene ID
29384
Gene Name (Vector)
H2A histone family, member Y
Gene Symbol
H2afy
ORF Size (aa)
1116
RefSeq ID
NM_017182
RefSeq Synonyms
NM_017182, BC089093,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.