mouse CD274 shRNA silencing AAV

Name 
AAV-m-CD274-shRNA
Cat No 
shAAV-280624
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Cd274.

shAAV-280624
AAV-m-CD274-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Cd274 (CD274 antigen). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A530045L16Rik; B7 homolog 1; B7h1; B7-H1; CD antigen CD274; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; programmed cell death 1 ligand 1; Programmed death ligand 1
Description (eCommerce)
PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].
Description (Vector)
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Gene ID
60533
Gene Name (eCommerce)
CD274 antigen
Gene Name (Vector)
CD274 antigen
Gene Symbol
Cd274
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
873
ORF Size (bp)
873 bp
Protein Name (eCommerce)
Programmed cell death 1 ligand 1
RefSeq ID
NM_021893
RefSeq Synonyms
NM_021893, BC066841,
Research Areas
Immune Cells and Tumor Markers,Immune-checkpoints,Immunology,Immuno-Oncology,Signal Transduction
Research Areas (Faceted)
signal_transduction,immuno_oncology,immunology,immuno_oncology
Species
mouse
Target Sentence
PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].
UniGene ID
Mm.245363
UniProt ID (eCommerce)
Q9EP73

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.