mouse POLR3A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Polr3a.
shAAV-278614
AAV-m-POLR3A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Polr3a (polymerase (RNA) III (DNA directed) polypeptide A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
9330175N20Rik; BC053071; RPC1; RPC155
Description (Vector)
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Gene ID
218832
Gene Name (Vector)
polymerase (RNA) III (DNA directed) polypeptide A
Gene Symbol
Polr3a
ORF Size (aa)
4173
ORF Size (bp)
4173 bp
RefSeq ID
NM_001081247
RefSeq Synonyms
NM_001081247, BC151169, BC151168, BC151002,
Species
mouse
UniGene ID
Mm.343610
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.