mouse WTIP shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Wtip.
shAAV-276349
AAV-m-WTIP-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Wtip (WT1-interacting protein). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. May monitor slit diaphragm protein assembly, a specialized adherens junction characteristic of podocytes. In case of podocyte injury, it shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype.
Gene ID
101543
Gene Name (Vector)
WT1-interacting protein
Gene Symbol
Wtip
ORF Size (aa)
1197
ORF Size (bp)
1197 bp
RefSeq ID
NM_207212
RefSeq Synonyms
NM_207212, BC054125,
Species
mouse
UniGene ID
Mm.422738
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.