mouse VPS25 shRNA silencing AAV

Name
AAV-m-VPS25-shRNA

Cat No
shAAV-276050

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Vps25.

shAAV-276050

AAV-m-VPS25-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Vps25 (vacuolar protein sorting 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

1110020N13Rik; AW107467; D11Wsu68e

Description (Vector)

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Gene ID

28084

Gene Name (Vector)

vacuolar protein sorting 25

Gene Symbol

Vps25

ORF Size (aa)

531

ORF Size (bp)

531 bp

RefSeq ID

NM_026776

RefSeq Synonyms

NM_026776, NM_001284414, NM_001284412, NM_001284411, BC029181,

Species

mouse

UniGene ID

Mm.301020

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.