mouse USP9Y shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Usp9y.
shAAV-275761
AAV-m-USP9Y-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Usp9y (ubiquitin specific peptidase 9, Y chromosome). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Dffry; Fafl2
Description (Vector)
USP9Y may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X re-empowers its competence to mediate TGF-beta signaling (By similarity).
Gene ID
107868
Gene Name (Vector)
ubiquitin specific peptidase 9, Y chromosome
Gene Symbol
Usp9y
ORF Size (aa)
7668
ORF Size (bp)
7668 bp
RefSeq ID
NM_148943
RefSeq Synonyms
NM_148943, BC172601,
Species
mouse
UniGene ID
Mm.347376
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.