mouse UCHL1 shRNA silencing AAV

Name 
AAV-m-UCHL1-shRNA
Cat No 
shAAV-275559
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Uchl1.

shAAV-275559
AAV-m-UCHL1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Uchl1 (ubiquitin carboxy-terminal hydrolase L1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AW822034; C88048; gad; PGP 9.5; PGP9.5; R75593; UCH-L1; UCHL-1
Description (Vector)
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Gene ID
22223
Gene Name (Vector)
ubiquitin carboxy-terminal hydrolase L1
Gene Symbol
Uchl1
ORF Size (aa)
672
RefSeq ID
NM_011670
RefSeq Synonyms
NM_011670, BC039177,
Species
mouse
UniGene ID
Mm.29807

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.