mouse SUV420H1 shRNA silencing AAV

Name 
AAV-m-SUV420H1-shRNA
Cat No 
shAAV-273487
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Kmt5b.

shAAV-273487
AAV-m-SUV420H1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Kmt5b (lysine methyltransferase 5B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AA117471; C630029K18Rik; Suv4-20h1; Suv420h1
Description (Vector)
Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity).
Gene ID
225888
Gene Name (Vector)
lysine methyltransferase 5B
Gene Symbol
Kmt5b
ORF Size (aa)
2454
ORF Size (bp)
2454 bp
RefSeq ID
NM_144871
RefSeq Synonyms
NM_144871, NM_001167889, NM_001167888, NM_001167887, NM_001167886, NM_001167885, NM_001167884, BC075709,
Species
mouse
UniGene ID
Mm.395622

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.