mouse RNF31 shRNA silencing AAV

Name
AAV-m-RNF31-shRNA

Cat No
shAAV-270739

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Rnf31.

shAAV-270739

AAV-m-RNF31-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rnf31 (ring finger protein 31). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AL033293; BC031509; Flj10111; HOIP; mFLJ00217; Paul

Description (Vector)

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.

Gene ID

268749

Gene Name (Vector)

ring finger protein 31

Gene Symbol

Rnf31

ORF Size (aa)

3201

ORF Size (bp)

3201 bp

RefSeq ID

NM_194346

RefSeq Synonyms

NM_194346, BC057595,

Species

mouse

UniGene ID

Mm.23748

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.