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mouse RNF135 shRNA silencing AAV

Name 
AAV-m-RNF135-shRNA
Cat No 
shAAV-270688
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Rnf135.

shAAV-270688
AAV-m-RNF135-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rnf135 (ring finger protein 135). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
0610037N03Rik; 2410006N06Rik
Description (Vector)
Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.
Gene ID
71956
Gene Name (Vector)
ring finger protein 135
Gene Symbol
Rnf135
ORF Size (aa)
1254
ORF Size (bp)
1254 bp
RefSeq ID
NM_028019
RefSeq Synonyms
NM_028019, BC138303, BC132307, BC096385,
Species
mouse
UniGene ID
Mm.22985

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.