mouse RIPK2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Ripk2.
shAAV-270613
AAV-m-RIPK2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Ripk2 (receptor (TNFRSF)-interacting serine-threonine kinase 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2210420D18Rik; CARD3; CARDIAK; CCK; D4Bwg0615e; RICK; RIP2
Description (Vector)
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Gene ID
192656
Gene Name (Vector)
receptor (TNFRSF)-interacting serine-threonine kinase 2
Gene Symbol
Ripk2
ORF Size (aa)
1620
ORF Size (bp)
1620 bp
RefSeq ID
NM_138952
RefSeq Synonyms
NM_138952, NM_001329751, BC069878,
Species
mouse
UniGene ID
Mm.112765
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.