mouse QTRT1 shRNA silencing AAV

Name 
AAV-m-QTRT1-shRNA
Cat No 
shAAV-269929
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Qtrt1.

shAAV-269929
AAV-m-QTRT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Qtrt1 (queuine tRNA-ribosyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2610028E17Rik; Tgt
Description (Vector)
Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).
Gene ID
60507
Gene Name (Vector)
queuine tRNA-ribosyltransferase 1
Gene Symbol
Qtrt1
ORF Size (aa)
1212
ORF Size (bp)
1212 bp
RefSeq ID
NM_021888
RefSeq Synonyms
NM_021888, BC044811,
Species
mouse
UniGene ID
Mm.436579

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.