mouse PUS1 shRNA silencing AAV

Name 
AAV-m-PUS1-shRNA
Cat No 
shAAV-269860
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Pus1.

shAAV-269860
AAV-m-PUS1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Pus1 (pseudouridine synthase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A730013B20Rik; MPUS1; mPus1p
Description (Vector)
Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (By similarity).
Gene ID
56361
Gene Name (Vector)
pseudouridine synthase 1
Gene Symbol
Pus1
ORF Size (aa)
1272
ORF Size (bp)
1272 bp
RefSeq ID
NM_019700
RefSeq Synonyms
NM_019700, NM_001347390, NM_001025562, NM_001025561, BC034359, BC021446,
Species
mouse
UniGene ID
Mm.474515

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.