mouse PKM2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Pkm.
shAAV-268736
AAV-m-PKM2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Pkm (pyruvate kinase, muscle). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2
Description (Vector)
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Gene ID
18746
Gene Name (Vector)
pyruvate kinase, muscle
Gene Symbol
Pkm
ORF Size (aa)
1596
ORF Size (bp)
1596 bp
RefSeq ID
NM_011099
RefSeq Synonyms
NM_011099, NM_001253883, BC094663, BC016619,
Species
mouse
UniGene ID
Mm.326167
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.