mouse PECAM1 shRNA silencing AAV

Name
AAV-m-PECAM1-shRNA

Cat No
shAAV-268377

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Pecam1.

shAAV-268377

AAV-m-PECAM1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Pecam1 (platelet/endothelial cell adhesion molecule 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

C85791; CD antigen CD31; Cd31; Pecam; PECAM-1; platelet endothelial cell adhesion molecule

Description (eCommerce)

CD31 is a cell adhesion molecule expressed mainly in platelets, endothelial cells, and a subset of leukocytes. CD31 localizes to cell-cell junctions and functions as a signaling molecule involved in a variety of vascular-related processes such as angiogenesis, platelet function, and inflammation.

Description (Vector)

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Gene ID

18613

Gene Name (eCommerce)

platelet/endothelial cell adhesion molecule 1

Gene Name (Vector)

platelet/endothelial cell adhesion molecule 1

Gene Symbol

Pecam1

NCBI Taxonomy ID (eCommerce)

10090.0

ORF Size (aa)

2094

ORF Size (bp)

2094 bp

Protein Name (eCommerce)

Platelet endothelial cell adhesion molecule

RefSeq ID

NM_008816

RefSeq Synonyms

NM_008816, NM_001305158, NM_001305157, NM_001032378, BC085502, BC008519,

Research Areas

Cardiovascular,Immune Cells and Tumor Markers,Immunology,Immuno-Oncology,Signal Transduction,T-cell Receptor Signaling

Research Areas (Faceted)

cardiovascular,immunology,immuno_oncology,signal_transduction

Species

mouse

Target Sentence

UniGene ID

Mm.343951

UniProt ID (eCommerce)

Q08481

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.