mouse NPHP1 shRNA silencing AAV

Name
AAV-m-NPHP1-shRNA

Cat No
shAAV-265982

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Nphp1.

shAAV-265982

AAV-m-NPHP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nphp1 (nephronophthisis 1 (juvenile) homolog (human)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis (By similarity).

Gene ID

53885

Gene Name (Vector)

nephronophthisis 1 (juvenile) homolog (human)

Gene Symbol

Nphp1

ORF Size (aa)

2058

ORF Size (bp)

2058 bp

RefSeq ID

NM_016902

RefSeq Synonyms

NM_016902, NM_001291013, NM_001291012, BC118953,

Species

mouse

UniGene ID

Mm.477771

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.